Evaluates the ability to design PCR primers for site-directed mutagenesis by analyzing plasmid sequences and…
Evaluates the ability to design PCR primers for site-directed mutagenesis by analyzing plasmid sequences and…: a task in terminal-bench-2-1-apptainer-v1: Terminal-Bench 2.1 (offline Apptainer, v1) (Harbor dataset). The file titled sequences.fasta contains the sequence for a circular input plasmid…
The task
The file titled sequences.fasta contains the sequence for a circular input plasmid, and a desired output plasmid. Design primers so that the input plasmid will be converted to the output plasmid when using NEB's Q5 site-directed mutagenesis kit. The primers should respect the following rules: * The part of the…